Isolation of Ventricular Myocytes from Adult Rat - adult rat cardiac myocytes


adult rat cardiac myocytes

Single ventricular myocytes were isolated from adult male Sprague-Dawley rats ( – g) as described previously. Briefly, hearts were rapidly removed and perfused (at 37°C) with oxygenated (5% CO 2 % O 2) Krebs-Henseleit bicarbonate (KHB) buffer (m M): NaCl , KCl , CaCl 2 , MgSO 4 , KH 2 PO 4 , NaHCO 3 25, N-[2-hydro-ethyl]-piperazine-N′-[2-ethanesulfonic acid] Cited by:

Cardiac myocytes isolated from adult rat hearts were grown on laminln coated culture dishes for more than a month. During this tune, the cells underwent a morphological transformation which has also been referred to by others as cell remodeling (Guo J-X, Jacobson SL, Brown DL.

Aug 01,  · Electrophysiological properties of adult rat ventricular myocytes cultured in Medium with Earle's salts (without glutamine) supplemented with 25 mM HEPES and NaHCO 3, 2 mg ml −1 bovine serum albumin, 2 mM l -carnitine, 5 mM taurine, 5 mM creatine, μM insulin and 10 −10 M 3,5,3″ by:

Male Sprague-Dawley rats weighing ~ g are commonly used. Female rats and other strains of rats can also be used for isolation of cardiac myocytes. After overnight fasting, the rats will be treated with heparin (50 units/ g body weight, intraperitoneal injection) .